p egfr y992  (Cell Signaling Technology Inc)

Journal: Frontiers in Oncology

Article Title: The Combination of Gefitinib With ATRA and ATO Induces Myeloid Differentiation in Acute Promyelocytic Leukemia Resistant Cells

doi: 10.3389/fonc.2021.686445

Figure Lengend Snippet: EGFR, p-EGFR (Tyr992), SYK, EGF, and AREG protein expression in APL patient samples. (A) Western blotting analysis of EGFR, p-EGFR (Tyr992), SYK, and β-actin protein levels in CD34 + cells isolated from normal BM of one healthy adult volunteer and 10 representative primary APL samples collected at diagnosis. HeLa cells served as a positive control to assess EGFR and p-EGFR (Tyr992) expression, and MV4-11 cell extracts were used as a positive control for SYK expression. EGFR and p-EGFR (Tyr992) were detectable when exposed for 180 seconds (sec). (B) EGF levels in BM plasma samples from healthy donors (n=15) and APL patients at diagnosis (n=16). (C) Plasma AREG levels in BM from healthy donors (n=20) and APL patients at diagnosis (n=17). (**) p < 0.01 (Mann-Whitney U-test).

Article Snippet: The proteins were transferred to a polyvinylidene difluoride membrane (Amersham Hybond-P; GE Healthcare, Memphis, TN, USA) that was probed with antibodies against total EGFR (#2232, polyclonal, 1:1000) and phosphorylated (p-)EGFR (Tyr992) (#2235, polyclonal, 1:1000) (both from Cell Signaling Technology, Danvers, MA, USA); SYK (#1240, clone: 4D10, 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA); and β-actin (#A5441, clone: AC-15, 1:60 000; Sigma-Aldrich).

Techniques: Expressing, Western Blot, Isolation, Biomarker Discovery, Positive Control, Clinical Proteomics, MANN-WHITNEY

Journal: Cell reports

Article Title: Small-molecule and CRISPR screening converge to reveal RTK dependencies in pediatric rhabdoid tumors

doi: 10.1016/j.celrep.2019.07.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: p-EGFR (Tyr992) , Cell Signaling , Cat#2235; RRID: AB_331708.

Techniques: Recombinant, Cell Viability Assay, Protease Inhibitor, Injection, Sample Prep, Expressing, Activity Assay, Plasmid Preparation, Software, Quantitative Proteomics, Methylation, Membrane

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Pantoprazole pretreatment elevates sensitivity to vincristine in drug-resistant oral epidermoid carcinoma in vitro and in vivo.

doi: 10.1016/j.biopha.2019.109478

Figure Lengend Snippet: Fig. 7. Combination treatment of PPZ and VCR downregulates EGFR/MAPK and PI3K/Akt/ mTOR signaling pathways in KB/V cells. a and b. The expression levels of EGFR, p-EGFR, p-ERK and p-p38 were detected and quantified by Western blotting assay. c Representative blots of PI3K, Akt, p-Akt, mTOR, and p-mTOR are shown. For a-c, data are presented as the mean ± SD from three separate experiments. *P < 0.05, **P < 0.01 vs. KB/V control group, #P < 0.05, ##P < 0.01 vs. VCR alone group.

Article Snippet: Membranes were blocked in 5% (w/v) non-fat milk before incubation with primary antibodies (1:1000 in primary antibody diluent) against PARP (#9532), cleavedcaspase 3 (#9664), Bcl-xL(#2762), Bcl-2 (#2872), Bax (#2772), p21 (#2947), Cyclin B1 (#4138), cdc2 (#9112), p-cdc2 (#9111), MMP2 (#4022), MMP9 (#3852), P-gp (#13342), EGFR (#2232), p-EGFR (#2235), p-ERK (#4370), p-p38 (#4511), PI3K (#3358), Akt (#2965), p-Akt (#2965), mTOR (#2972), p-mTOR (#2971), β-actin (#4970) (all from Cell Signaling Technology, CST, Boston, MA) and V-ATPase (14418-1-AP, Proteintech, Wuhan, China) at 4 °C overnight.

Techniques: Protein-Protein interactions, Expressing, Western Blot, Control

Journal: Biochimica et biophysica acta

Article Title: Role of MSC-derived Galectin 3 in the AML Microenvironment

doi: 10.1016/j.bbamcr.2018.04.005

Figure Lengend Snippet: List of antibodies used for RPPA

Article Snippet: EGFR-phospho Tyr992 , EGFR-p tyr992 , Cell Signaling , 2235 , Rabbit , 50.

Techniques: Staining, Transduction